Youth injections: the relationship between the concentration of hyaluronic acid and the effects of biorevitalization

An important role in skin hydration is played by hyaluronic acid (HA), which is one of the main components of the extracellular matrix of the skin.  Age-related changes in the skin largely depend on the qualitative and quantitative changes in HA from skin tissues.  In order to replenish the amount of HA in the skin, a biorevitalization procedure is carried out – intradermal injection of unmodified HA. Currently, various biorevitalizants are presented on the aesthetic market. HA stimulates the regeneration of the dermis, but the effect of its impact depends on many factors: on the molecular weight, origin and concentration of the active substance, the presence of additional ingredients in the preparation. Read about the relationship between the concentration of HA and the effects exerted when biorevitalizants are introduced into the dermis with mannitol, read on estet-portal. com in this article.  

Mannitol: is an antioxidant that can protect HA from free radical damage. The authors of a number of scientific publications cite evidence that mannitol, as part of biorevitalizants, slows down the degradation of HA, helps to reduce the oxidative modification of skin proteins, which significantly increases the duration of the drug and reduces the number of necessary procedures.

Hyaluronic acid for the skin: optimal concentration

A hot topic is the effect of HA concentration on cell vitality and the effectiveness of the biorevitalization procedure. There is evidence of a decrease in the metabolic activity of fibroblasts and a decrease in the level of skin hydration with intradermal injections of highly concentrated HA in the first few days after drug administration.  In studies conducted at a later date after the administration of drugs, it is indicated that the revitalizing effect of HA was manifested when its concentration decreased to physiological values, in the process of natural biodegradation.

Efficacy and safety studies of unmodified HA at various concentrations

In order to study the effectiveness and safety of the use of unmodified HA of various concentrations, when conducting intradermal injections, the scientific supervisor of OKK-EXPERT Ph.D. Larkina Svetlana with co-authors conducted morphological studies of the skin of experimental animals (rabbits).  The studies were carried out on the basis of the histological laboratory of the Department of Pathological Anatomy with a sectional course of the Odessa National Medical University. For the study, 4 biorevitalizants with different concentrations of HA containing mannitol were selected: Stylage Hydro and Luminera Hydryal 2%, 3%, 4%.

After the administration of biorevitalizants with mannitol containing various concentrations of HA, the following results were observed (skin samples were taken after 2 weeks, 1 and 2 months after injections):

• Morphological changes were observed in all samples.
• No degenerative or atrophic processes were found.
• There was an increase in the number of connective tissue fibers in all layers of the dermis and in all samples, compared with the control preparation.
• When using 1.4% and 2% HA solution, more pronounced changes were observed in the papillary layer. When using a 3% and 4% solution of hyaluronic acid — changes were expressed in all layers of the dermis, but to a greater extent in the reticular layer.
• 2 weeks after administration of 4% HA solution: an increase in the number of fibroblasts in the papillary dermis was noted, almost 4 times compared with the control sample.
• Drugs with a lower concentration of HA (1.4% and 2%) gave more pronounced results in the early stages (2 - 4 weeks), and drugs with a high concentration of HA (3.0% and 4.0%) — at a later date (8 weeks).

Drugs with a lower concentration of HA (1.4% and 2%) give more pronounced results in the early stages (2 - 4 weeks), and drugs with a high concentration of HA (3.0% and 4.0%) — at a later date (8 weeks). 

The conducted studies indicate that the formation of connective tissue fibers of the dermis increased with an increase in the concentration of hyaluronic acid, which may indicate a greater compaction of the dermis when using highly concentrated biorevitalizants. It is also possible to assume the relationship between the concentration of HA and morphological changes in the skin: the higher the concentration, the later the biorevitalizing effect occurs, which may indicate that the stimulating effect of HA occurs when its physiological concentration in tissues is reached. The antioxidant activity of mannitol provides a slowdown in the biodegradation of HA, which may serve as a basis for reducing the number of necessary procedures.

Mannitol, included in all Luminera Hydyral booster-revitalizers, protects hyaluronic acid from free radicals, which ensures a longer effect from the use of drugs.

Luminera Hydryal is a line of unique HA preparations in combination with the antioxidant mannitol, which provides prolonged revitalization, restoration of water balance and skin structure.

Exclusive representative of Luminera Hydryal booster-revitalization products in Ukraine and Moldova: OKK-EXPERT PHARMA COMPANY.